Experimental Design
Purpose: What is the affect of acid rain on the photosynthesis rate of primroses?
Hypothesis: If you water the primrose with acid then it will slow down the oxygen production of the primrose.
Materials:
1. 6 Primroses
2. Acid rain
3. 6 Plastic containers
4. Oxygen probe
5. 2 Syringes
6. 6 stopper
7. Plain water
8. Goggles
9. Apron
Independent Variables:
The ph level of the acid rain
Dependent Variable:
The oxygen production of the primrose
Procedure:
1. Remove the flowers off of three of the primroses
2. Put the primroses with flowers in three separate plastic container
3. Put the primroses with no flowers in three separate containers
4. Tape every container shut and stick the stoppers in the hole
5. Put 100 ml of plain water into the syringe
6. Quickly take the stopper out the container with the primrose without flowers
7. Stick the syringe into the hole in the container
8. Squeeze out the water into the soil of the primrose
9. Quickly take the syringe out the container, and then put the stopper back in it
10. Put 100 ml of water into the same syringe
11. Quickly take the stopper out the container with the primroses with flowers
12. Stick the syringe in the hole in the container
13. Squeeze the water out into the soil of the primrose
14. Take the syringe out and put the stopper back in the container
15. Take one of the containers with a primrose with no flowers
16. Put 100 ml of acid rain with a pH of 4 in a different syringe
17. Quickly take the stopper out the container
18. Squeeze the acid rain into the soil of the primrose
19. Take the syringe out of the container and put the stopper back in
20. Get the container of primroses with flowers
21. Put 100 ml of acid rain with a pH of 4 in a different syringe
22. Take the stopper out the container
23. Squeeze the acid rain out into the soil of the primrose
24. Quickly take the syringe out of the container and put the stopper back in
25. Repeat steps 15-24 exactly but water the primroses with acid rain that has a pH of 3 instead of 4
26. Take a picture of the setup
27. After 24 hours test the oxygen production using a oxygen probe
28. Stick the oxygen probe into the hole in the container
29. Remove the oxygen probe from the container after it collects the data
30. Stick the oxygen probe into the computer and open data studio
31. Repeat everyday for 2 weeks
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